Size-selective microplastic uptake by freshwater organisms: Fish, mussel, and zooplankton.

Microplastics, as an emergent pollutant, have garnered substantial attention within aquatic environments, yet a significant knowledge gap persists regarding the interplay of organism size and pollution impacts on microplastic uptake in freshwater ecosystems. The main aim of the current study is to assess the microplastic ingestion by aquatic organisms across diverse trophic levels. To achieve this objective, zooplankton, mussels (Anodonta anatina), and fish (Carassius gibelio) were collected from the highly polluted Susurluk River Basin in Türkiye. The size distribution encompassed 160.8 ± 56.9 µm for the prevailing zooplankton, 6.9 ± 2.2 cm for mussel, and 20.4 ± 3.1 cm for fish, respectively. While no microplastic ingestion was observed among zooplankton, the finding highlights the influence of body-size and pollution on microplastic ingestion. In contrast, A. anatina and C. gibelio contained 617 and 792 microplastic particles, respectively. Predominantly, fibers emerged as the most prevalent microplastic type across trophic levels (except zooplankton) followed by films. Notably, only fish exhibited fragments within their gastrointestinal tract. A substantial correlation emerged between microplastic abundance and mussel size and weight, but no such correlation manifested for fish. The study also revealed a positive link between microplastic count and turbidity (phosphate and high Chl a level), impacting mussel ingestion capacity due to the variability in the food availability and potential shifts in feeding preferences. Conversely, no distinct pattern emerged for fish concerning water quality parameters and ingested microplastics. Consequently, our study underscores diverse microplastic uptake patterns in freshwater ecosystems, with a predominant frequency of microplastics falling with the 0.3 mm-3.0 mm range, emphasizing the significance of size-selective uptake by organisms.

Tracking the microplastic accumulation from past to present in the freshwater ecosystems: A case study in Susurluk Basin, Turkey.

Microplastic pollution in aquatic ecosystems has become a global issue in recent years due to its presence everywhere around the world. Although several studies have explored the impact of the accumulation of those small particles in marine environments, comparisons of freshwater systems with marine environments are scarce. In the current study, due to the lack of long-term data on microplastic pollution, we used paleolimnological approaches to acquire the missing information regarding this hot topic. Two short cores were taken from Bursa province in Turkey, which is the center of industrial and agricultural production with many different sectors such as textile and manufacturing. The first core sample was taken from a relatively pristine environment, Lake Uluabat, and the second one was taken from a delta area where all the discharge coming from the basin flowed through to the Marmara Sea. The sediment core from the lake was dated back to the 1960's and the majority of the sample was dominated by fibers. Despite there being no uniform distribution pattern, the number of the microplastics showed decreasing trend after the lake became a Ramsar site. Due to the continuous mixing in the sampling area, there were obstacles via the dating of the Delta core. Nevertheless, the data showed that a high number and variety of microplastics have accumulated over the last decade in the province. This can be interpreted as microplastic pollution reaching the sea directly from the basin. These findings revealed that a plastic chronostratigraphy would give important temporal data regarding the microplastic accumulation in aquatic ecosystems.

A review of plastic pollution in aquatic ecosystems of Turkey.

Turkey is one of the major plastic pollution sources in the Mediterranean and the Black Sea. This review summarizes present information, data, and legislation on plastic pollution in Turkish aquatic ecosystems. According to results derived from reviewed studies, both macro- and microplastic pollutions were documented in Turkish aquatic ecosystems. Most of the studies on plastic pollution in Turkish waters were performed in the marine environment while only four were conducted in freshwater environments. Spatially, the majority of these studies, which were on levels in the marine environment, were conducted on the northeastern Mediterranean coasts of Turkey, especially Iskenderun and Mersin Bays. Additional studies were carried out on either the ingestion/presence/impact of microplastics by/to aquatic organisms or the entanglement of marine organisms in plastics. There were also studies assessing the microplastic content of commercial salt, and another has reported microplastic presence in traditional stuffed mussels sold in Turkish streets. Some studies were conducted on microplastic presence and/or their removal in wastewater treatment plants in Mersin, Adana, Mugla, and Istanbul cities. Macro- and microliter loading from a few Turkish rivers to the sea was also estimated. All these investigations indicate that Turkish aquatic environments have significant plastic pollution problems, which were also underlined by the legislative studies. The need for further studies in this field still exists, especially in freshwater environments.

Microplastics in a dam lake in Turkey: type, mesh size effect, and bacterial biofilm communities.

The evaluation of microplastic (MP) pollution has been drawing attention for the last decades. MP pollution has been studied widely in marine environments, but limited data exists for freshwater ecosystems on potential source and transport of MPs. The type, shape, plastic components, and the color of the MPs were investigated using various-mesh-sizes (300 and 100 µm) nets in four sampling stations of Süreyyabey Dam Lake in Turkey. The growth of bacterial isolates on the MPs surface and surrounding water was also investigated. The type of the MPs and the interaction between the mesh size and the type of the MPs showed significant differences (p < 0.05). Fibers were found to be the most abundant particle type constituting 45% and 80% of the total MPs found in 330-µm and 100-µm mesh sizes, respectively. In total the observed MP abundance in the dam lake was 5.25 particles m-3, and 4.09 particles m-3 was observed for 100-µm and 330-µm mesh sizes, respectively. The color of the identified microplastics showed variations among microplastic types; however, the dominant color was transparent in each net. The main plastic components of the MPs are polyethylene terephthalate, polyvinyl chloride, polystyrene, polyethylene, and polypropylene. The microbial community mainly consists of potentially pathogenic strains such as Escherichia coli, Enterococcus faecalis, and Acinetobacter baumanii complex. The current study could contribute valuable background information both for MP pollution and for biofilm composition in a dam. However, the surface of the MPs and biofilm formation should be investigated urgently to understand the vector potential of MPs.

Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins.

Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.